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Project Outcomes

Category: Project Outcomes

DeDNAed Final Webinar Recording

On 17 July 2024, the DeDNAed partners gathered online for our final dissemination webinar, entitled “Uniting disciplines: How DeDNAed combines physics and biology for novel biosensor development”.

Coordinator Danny Reuter (Technical University of Chemnitz) welcomed the participants and provided an overview of the DeDNAed project, its goals and consortium. Then Andreas Heerwig of the Kurt-Schwabe-Institut fÞr Mess- und Sensortechnik Meinsberg e.V. (KSI Meinsberg), explained “DNA origami as a tool for precise positioning of sensing elements towards quantitative SERS measurements”.

This was followed by our CIC biomaGUNE partner Itziar Galarreta-Rodriguez, who spoke about “ssDNA aptamers as biorecognition elements for DNA origami biosensor application”. Staying in San Sebastian, we then jumped to partner TECNALIA: VerÃģnica Mora Sanz explained “Antibody-protected bimetallic nanoclusters as biorecognition and transduction element in a DNA-origami based biosensor”.

The first section closed with Saloni Agarwal of University of Potsdam, speaking about “Characterising binding affinity of bio-recognition elements and their targets using switchSENSE technology.”

After a short break, we returned to Julia Voglhuber-HÃķller of BioNanoNet Forschungsgesellschaft mbH (BNN), who outlined “How Safe(-and-Sustainable)-by-Design can support innovations in the biosensor field”. She was followed by DeDNAed project manager Julia Hann, also of TU Chemnitz, who spoke about “Spatially resolved integration of heterogeneous DNA origami nanosystems on nanostructured surfaces for SERS”.

Finally, Aicha Azziz of the University of Le Mans explained “SERS detection of benzophenone using DNA origami hybrids”. The webinar was moderated by Caitlin Ahern of BNN.

The webinar attracted 50 live participants and ended with a Q&A session.

The recording can be viewed below!

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Coordinator TU Chemnitz integrates DNA origami hybrids

In addition to coordinating the DeDNAed project, TU Chemnitz leads the “Integration of DNA origami hybrids” and “Transfer to flexible substrates and biosafety”. They are a bridge between the nanocomponents.

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BNN leads Safe-by-Design and dissemination activities for DeDNAed

DeDNAed partner BNN generates a Safe-by-Design (SbD) concept for the sensor platform. In addition, BNN manages the communication and dissemination activities for the H2020 project.

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TECNALIA works with nanoclusters and plans the exploitation of DeDNAed

DeDNAed partner TECNALIA is working with antibodies decorated with nanoclusters as well as nanobodies. They also lead the exploitation of the project.

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IMMM characterizes nanoparticles using SERS

The Institute of Molecules and Materials of Le Mans (IMMM) is in charge of the characterization of the optical and plasmonic properties of the nanostructures included in the bioanalytical platform. It also determines the SERS efficiency and the sensing performances of the platform. Finally, it is involved in the validation of the sensor for food and biomedical applications.

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CIC biomaGUNE creates plasmonic particles and sensing elements

DeDNAed partner CIC biomaGUNE is responsible for design, synthesis, and characterization of different sensing biorecognition elements (antibodies and DNA aptamers) in pure state and modified with metallic and semiconductor atomic clusters. They also design, synthesize, and provide metallic nanoparticles of different size and shape as amplifiers of optical read out signals.

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First Joint Publication for DeDNAed

In the field of medical diagnostics, the identification and measurement of biomarkers have become essential tools for early disease detection and treatment monitoring.

Within DeDNAed, Le Mans UniversitÃĐ (France) and the research centre TECNALIA Research & Innovation (Spain) focus their research on the detection of interleukin-6, a cytokine which can be used to monitor various human diseases and treatment responses. Conventional assays, such as ELISAs, are time-consuming and require large sample volumes. Therefore, the development of a fast, sensitive and selective detection method is critical.

Researchers from DeDNAed proposed a sensing method based on the surface-enhanced Raman scattering (#SERS), a technique that emerges as a promising alternative for the highly sensitive IL-6 detection.

They developed a label-free SERS immunoassay, achieving an impressive limit of detection of 0.91 pg mL−1 (0.04 pM), ten times lower than ELISA. Moreover, this label-free approach offers a simple, and fast (~90 min) assay for direct IL-6 monitoring.

This study, “Label-free SERS for rapid identification of interleukin 6 based on intrinsic SERS fingerprint of antibody‑gold nanoparticles conjugate,” published in December 2023 in the International Journal of Biological Macromolecules, showcased the collaborative success between the University of Le Mans and the research centre TECNALIA thanks to authors Marjan Majdinasab, Aicha Azziz, Qiqian Liu, VerÃģnica Mora-Sanz, Nerea Briz, Mathieu Edely and Marc Lamy de la Chapelle.

Read the publication here.

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